Dr. B. Das

Expertise: Microbiology, Molecular Biology and Cell Biology

Ph. D.,  MSc. and BSc. in Microbiology

Research Interest:

General Microbiology
 Isolation of microbes from soil and other natural sources
 Stock maintenance, subculturing
 Staining
 Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of antimicrobial agents (Zone of inhibition in agar diffusion method, Broth dilution shaking method) Molecular Biology and cell biology techniques
 Gene cloning and Polymerase chain reaction (PCR)
 Gene deletion in S.cerevisiae
 siRNA mediated gene knockdown
 DNA and RNA Isolation from both prokaryotes and eukaryotes cells.
 cDNA synthesis and quantitative PCR
 Transformation in S.cerevisiae and bacteria
 DNA sequencing
 Cell viability assays – Tunnel assay, Dual staining, MTT assay
 ROS detection in cells by FACS Protein biochemistry techniques
 Protein overexpression and purification
 Protein electrophoresis
 Western-blotting
 Immunoprecipitation
 Immunofluorescence and Immunohistochemistry in animal tissue sections using Fluorescence and Confocal microscope, Experimental Animal Models
 Experienced in handling of guinea pig, Cell/Tissue Culture Techniques
 Mammalian adherent and nonadherent cell culture
 Transfection and stable cell line generation, Bioinformatics, and statistics:
 BLAST, Pairwise, and multiple sequence alignment.
 Retrieval of data from different genomic and protein databases, domain analysis, etc
 Test of variance, ANOVA, Test of significance.

 

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