High Throughput Screening
It is an automated drug discovery process which helps to find active compounds or leads quickly. A large number of compounds can be screened and different tests like chemical, genetic or pharmacological can be performed by use of robots and software control.
The samples which are showing some positive results are referred as hits then assayed further to confirm and redefine the finding.
The main feature of HTS is its automation with robotics which allows mixing, incubation, and detection. About 100,000 compounds are screened in a single day. There are HTS robots which screen more than 100,000 compounds per day, and this is referred as ultra-high throughput screening. Quality control is maintained both experimentally as well as with computational approach which involves good plate design, selection of chemical controls, statistical knowledge to measure the degree of differentiation to exclude inferior data.
HTS has certain limitations like we cannot find out bioavailability, pharmacokinetics, toxicity and absolute specificity of the lead compound identified through it. It provides information about potency and activity. HTS has provided the drugs for many diseases as well as for their prevention for example Cyclosporin A, Mevastatin, etc.